Product nameAnti-PON2 antibody
See all PON2 primary antibodies
DescriptionRabbit polyclonal to PON2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog
- This antibody gave a positive signal in the following Human whole cell lysates: Caco 2; HeLa - Hydroxyurea Treated (48hr, 1µM). HeLa cell line in IF.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab40969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionCapable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
Tissue specificityWidely expressed with highest expression in liver, lung, placenta, testis and heart.
Sequence similaritiesBelongs to the paraoxonase family.
modificationsThe signal sequence is not cleaved.
- Information by UniProt
- A esterase 2 antibody
- A-esterase 2 antibody
- Aromatic esterase 2 antibody
Immunohistochemistical detection of PON2 antibody (ab40969) on formaldehyde fixed paraffin-embedded mosue kidney and lung sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/2000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated to Biotin (1/200). Patterns of immunostaining are produced that appear to be very similar to those seen on the HPA website, for these tissues. In the submitted composite image, both tissues show intense labelling. In the upper image of kidney cortico-medullary junction, apical membrane positivity is observed in proximal/distal tubules. In the same tubules globular intra-cytoplasmic positivity is also observed. In the lower image of lung, positivity is evident in the simple columnar epithelium of the bronchioles and also in what appear to be alveolar macrophages. This antibody
All lanes : Anti-PON2 antibody (ab40969) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 45 kDa (possible glycosylated form), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab40969 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Birkus G et al. Intracellular Activation of Tenofovir Alafenamide and the Effect of Viral and Host Protease Inhibitors. Antimicrob Agents Chemother 60:316-22 (2015). Read more (PubMed: 26503655) »
- Giordano G et al. Gender differences in brain susceptibility to oxidative stress are mediated by levels of paraoxonase-2 expression. Free Radic Biol Med 58:98-108 (2013). WB ; Mouse, Human . Read more (PubMed: 23376469) »