• Product name
  • Description
    Rabbit polyclonal to PON2
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41542.)

  • Positive control
    • This antibody gave a positive signal in the following Human whole cell lysates: Caco 2; HeLa - Hydroxyurea Treated (48hr, 1µM). HeLa cell line in IF.



Our Abpromise guarantee covers the use of ab40969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF Use a concentration of 5 µg/ml.



  • All lanes : Anti-PON2 antibody (ab40969) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 45 kDa (possible glycosylated form), 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 150 seconds

    Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunohistochemistical detection of PON2 antibody (ab40969) on  formaldehyde fixed paraffin-embedded mosue kidney and lung sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/2000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated to Biotin (1/200). Patterns of immunostaining are produced that appear to be very similar to those seen on the HPA website, for these tissues. In the submitted composite image, both tissues show intense labelling. In the upper image of kidney cortico-medullary junction, apical membrane positivity is observed in proximal/distal tubules. In the same tubules globular intra-cytoplasmic positivity is also observed. In the lower image of lung, positivity is evident in the simple columnar epithelium of the bronchioles and also in what appear to be alveolar macrophages. This antibody

    See Abreview

  • ICC/IF image of ab40969 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Birkus G  et al. Intracellular Activation of Tenofovir Alafenamide and the Effect of Viral and Host Protease Inhibitors. Antimicrob Agents Chemother 60:316-22 (2015). Read more (PubMed: 26503655) »
  • Giordano G  et al. Gender differences in brain susceptibility to oxidative stress are mediated by levels of paraoxonase-2 expression. Free Radic Biol Med 58:98-108 (2013). WB ; Mouse, Human . Read more (PubMed: 23376469) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your email and the additional information. This is very helpful.

I would suggest that if no protease inhibitors were used, to include them in your lysis buffer freshly, before lysing the cells (e.g. ab65621 protease Inhibitor cocktail, https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html). This is crucial to prevent degradation of proteins by proteases and other enzymes that are released from cell compartments during lysis.

What was the result for PON2 after 1/800 dilution. You can also use more antibody, but 1/800 is the suggested starting dilution.
For PON 3, please let me know your results as well (with 1/400).
Also, what bands did you obtain for the liver lysate?

In general, you can always increase the protein amount if the signal is weak as this can help with obtaining a band.

Is the secondary antibody working well with other primary antibodies?

Please let me know. If the results are still not satisfying, we can offer either free of charge replacements for each antibody, or a credit or refund. There will be no need to return the antibodies to us.

I look forward to hear back from you.

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Thank you for bringing this to our attention.

I am sorry to read that you are having difficulty obtaining a western blot signal. I may be able to make some recommendations but I will need a few more details about your samples and protocol.

Can you please tell me what kind of human tissues you are working with? How are the samples prepared? In particular, are they prepared with protease inhibitors? Are you confident that the PON proteins are expressed in these tissues?

How much of the sample do you load per lane of the gel?

Do you have evidence that the proteins transfer successfully from the gel to the membrane?

Have stained blots of the same samples for any other proteins?

For these two antibodies, we recommend a working solution of at least 1 ug/ml of antibody. The dilution factor will depend on the lot, but for the lots you have, the dilution of ab40969 that achieves 1 ug/ml is 1/800, and for ab42322, 1/400. incubating the blots with a more concentrated solution of antibody may help, but this assumes that at least 20ug of protein is loaded per lane. More important is the quality of the sample, and a positive control. For each of these proteins, a liver lysate will serve as a positive control. We also have purified PON2, ab53379.

I look forward to your reply.

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Thank you for contacting us.

We sell this product at a 100ul size. How many western blots you will be able to perform with that depends upon a number of factors including the concentration that is needed for your optimized protocol and the amount of primary antibody reagent you will be using. This has been tested at concentrations of 1ug of antibody per ml of incubation reagent, however your use may vary.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Kidney and lung)
Kidney and lung
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Nov 02 2010


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