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Goat polyclonal to PON2
This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).
Predicted to work with:
Rat, Dog, Pig
by a Cysteine residue linker, corresponding to internal sequence amino acids 93-103 of Human PON2 (NP_000296.2; NP_001018171.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
Widely expressed with highest expression in liver, lung, placenta, testis and heart.
Belongs to the paraoxonase family.
The signal sequence is not cleaved.
Information by UniProt
A esterase 2 antibody
A-esterase 2 antibody
Aromatic esterase 2 antibody
Western blot - Anti-PON2 antibody (ab99550)
Anti-PON2 antibody (ab99550) at 1 µg/ml (incubation time 1 hour) + HepG2 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
40 kDa (
why is the actual band size different from the predicted?
Additional bands at:
60 kDa. We are unsure as to the identity of these extra bands.
The 60kDa band was successfully blocked by incubation with the immunizing peptide.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"