Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PON2 antibody [EPR15295-82] (ab183710)

Reviews (3) Submit a question References (4)
Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Anti-PON2 antibody [EPR15295-82] (ab183710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15295-82] to PON2
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PON2 antibody [EPR15295-82]
    See all PON2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15295-82] to PON2

  • Host species

    Rabbit

  • Specificity

    Based on the sequence analysis, ab183710 recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A431 and U87-MG cell lysates. IHC-P: Human and Mouse kidney tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50 - 1/70.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50 - 1/70.

Target

  • Function

    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.

  • Tissue specificity

    Widely expressed with highest expression in liver, lung, placenta, testis and heart.

  • Sequence similarities

    Belongs to the paraoxonase family.

  • Post-translational
    modifications

    The signal sequence is not cleaved.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • A esterase 2 antibody
    • A-esterase 2 antibody
    • Aromatic esterase 2 antibody
    • Paraoxonase 2 antibody
    • Paraoxonase nirs antibody
    • PON 2 antibody
    • PON2 antibody
    • PON2_HUMAN antibody
    • Serum aryldialkylphosphatase 2 antibody
    • Serum paraoxonase/arylesterase 2 antibody
    see all

Images

  • Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PON2 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39,41 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39,41 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)

    Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2: TBS instead of HepG2 lysates.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-PON2 antibody [EPR15295-82] (ab183710)
    Anti-PON2 antibody [EPR15295-82] (ab183710)

References (4)

Publishing research using ab183710? Please let us know so that we can cite the reference in this datasheet.

ab183710 has been referenced in 4 publications.

  • Tao S  et al. Caspase-1-dependent mechanism mediating the harmful impacts of the quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone on the intestinal cells. J Cell Physiol 234:3621-3633 (2019). PubMed: 30471106
  • Faraz M  et al. A protein interaction network centered on leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) regulates growth factor receptors. J Biol Chem 293:3421-3435 (2018). PubMed: 29317492
  • Li W  et al. Paraoxonase 2 prevents the development of heart failure. Free Radic Biol Med 121:117-126 (2018). PubMed: 29729330
  • Shi S  et al. Regulation of the epithelial Na+ channel by paraoxonase-2. J Biol Chem 292:15927-15938 (2017). PubMed: 28768768

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1-3 of 3 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PON2 antibody [EPR15295-82]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Xenografted tumor intercranial)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Xenografted tumor intercranial
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Formaldehyde
Read More

Mr. Anton Poral

Verified customer

Submitted Jul 31 2019

IHC - Wholemount abreview for Anti-PON2 antibody [EPR15295]

 Excellent
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (normal and tumor sections)
Specification
normal and tumor sections
Read More

Abcam user community

Verified customer

Submitted Feb 27 2015

Western blot abreview for Anti-PON2 antibody [EPR15295]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T, mouse embryonic fibroblasts)
Specification
293T, mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 29 2014

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