Recombinant Anti-PON2 antibody [EPR15295-82] (ab183710)
![Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)](https://www.abcam.com/ps/products/183/ab183710/Images/ab183710-389429-anti-pon2-antibody-epr15295-82-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15295-82] to PON2
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PON2 antibody [EPR15295-82]
See all PON2 primary antibodies -
Description
Rabbit monoclonal [EPR15295-82] to PON2 -
Host species
Rabbit -
Specificity
Based on the sequence analysis, ab183710 recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.
Mouse species is recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse tissues. -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, A431 and U87-MG cell lysates. IHC-P: Human and Mouse kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15295-82 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).
Mouse species is recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse tissues. |
| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| IP |
1/50 - 1/70.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa). Mouse species is recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse tissues. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/50 - 1/70. |
Target
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Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. -
Tissue specificity
Widely expressed with highest expression in liver, lung, placenta, testis and heart. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5445 Human
- Entrez Gene: 330260 Mouse
- Entrez Gene: 296851 Rat
- Omim: 602447 Human
- SwissProt: Q15165 Human
- SwissProt: Q62086 Mouse
- SwissProt: Q6AXM8 Rat
- Unigene: 727553 Human
see all -
Alternative names
- A esterase 2 antibody
- A-esterase 2 antibody
- Aromatic esterase 2 antibody
see all
Images
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Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PON2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.
ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)](https://www.abcam.com/ps/products/183/ab183710/Images/ab183710-215733-anti-pon2-antibody-epr15295-82-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)](https://www.abcam.com/ps/products/183/ab183710/Images/ab183710-215731-anti-pon2-antibody-epr15295-82-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)](https://www.abcam.com/ps/products/183/ab183710/Images/ab183710-215734-anti-pon2-antibody-epr15295-82-western-blot.jpg)
Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)](https://www.abcam.com/ps/products/183/ab183710/Images/ab183710-215730-anti-pon2-antibody-epr15295-82-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2: TBS instead of HepG2 lysates.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-PON2 antibody [EPR15295-82] (ab183710)](https://www.abcam.com/ps/products/183/ab183710/Images/ab183710-6-benefits-of-recombinant-antibodies.png)
Anti-PON2 antibody [EPR15295-82] (ab183710)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (5)
ab183710 has been referenced in 5 publications.
- Xie F et al. Long non-coding RNA LINC00488 facilitates thyroid cancer cell progression through miR-376a-3p/PON2. Biosci Rep 41:N/A (2021). PubMed: 33600548
- Tao S et al. Caspase-1-dependent mechanism mediating the harmful impacts of the quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone on the intestinal cells. J Cell Physiol 234:3621-3633 (2019). PubMed: 30471106
- Faraz M et al. A protein interaction network centered on leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) regulates growth factor receptors. J Biol Chem 293:3421-3435 (2018). PubMed: 29317492
- Li W et al. Paraoxonase 2 prevents the development of heart failure. Free Radic Biol Med 121:117-126 (2018). PubMed: 29729330
- Shi S et al. Regulation of the epithelial Na+ channel by paraoxonase-2. J Biol Chem 292:15927-15938 (2017). PubMed: 28768768
