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  1. Link

    pon2-antibody-epr15295-82-ab183710.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PON2 antibody [EPR15295-82] (ab183710)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (5)

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Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)
  • Anti-PON2 antibody [EPR15295-82] (ab183710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15295-82] to PON2
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-SIRP alpha antibody [EPR16264] (ab191419)
Knockout
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Human PON2 knockout HeLa cell line (ab265927)

View more associated products

Overview

  • Product name

    Anti-PON2 antibody [EPR15295-82]
    See all PON2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15295-82] to PON2
  • Host species

    Rabbit
  • Specificity

    Based on the sequence analysis, ab183710 recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.
    Mouse species is recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse tissues.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A431 and U87-MG cell lysates. IHC-P: Human and Mouse kidney tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15295-82
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease

Associated products

  • Alternative Versions

    • Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (ab250686)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PON2 knockout HeLa cell line (ab265927)
  • KO cell lysates

    • Human PON2 knockout HeLa cell lysate (ab258128)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human PON2 protein (ab53379)
  • Related Products

    • Recombinant Human PON2 protein (ab53379)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).

Mouse species is recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse tissues.

IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50 - 1/70.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).

Mouse species is recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse tissues.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50 - 1/70.

Target

  • Function

    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
  • Tissue specificity

    Widely expressed with highest expression in liver, lung, placenta, testis and heart.
  • Sequence similarities

    Belongs to the paraoxonase family.
  • Post-translational
    modifications

    The signal sequence is not cleaved.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15165 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5445 Human
    • Entrez Gene: 330260 Mouse
    • Entrez Gene: 296851 Rat
    • Omim: 602447 Human
    • SwissProt: Q15165 Human
    • SwissProt: Q62086 Mouse
    • SwissProt: Q6AXM8 Rat
    • Unigene: 727553 Human
    • Unigene: 126984 Mouse
    • Unigene: 460999 Mouse
    • Unigene: 1100 Rat
    see all
  • Alternative names

    • A esterase 2 antibody
    • A-esterase 2 antibody
    • Aromatic esterase 2 antibody
    • Paraoxonase 2 antibody
    • Paraoxonase nirs antibody
    • PON 2 antibody
    • PON2 antibody
    • PON2_HUMAN antibody
    • Serum aryldialkylphosphatase 2 antibody
    • Serum paraoxonase/arylesterase 2 antibody
    see all

Images

  • Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PON2 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39,41 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
    All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39,41 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)
    Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)

    Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2: TBS instead of HepG2 lysates.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-PON2 antibody [EPR15295-82] (ab183710)
    Anti-PON2 antibody [EPR15295-82] (ab183710)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (5)

Publishing research using ab183710? Please let us know so that we can cite the reference in this datasheet.

ab183710 has been referenced in 5 publications.

  • Xie F  et al. Long non-coding RNA LINC00488 facilitates thyroid cancer cell progression through miR-376a-3p/PON2. Biosci Rep 41:N/A (2021). PubMed: 33600548
  • Tao S  et al. Caspase-1-dependent mechanism mediating the harmful impacts of the quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone on the intestinal cells. J Cell Physiol 234:3621-3633 (2019). PubMed: 30471106
  • Faraz M  et al. A protein interaction network centered on leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) regulates growth factor receptors. J Biol Chem 293:3421-3435 (2018). PubMed: 29317492
  • Li W  et al. Paraoxonase 2 prevents the development of heart failure. Free Radic Biol Med 121:117-126 (2018). PubMed: 29729330
  • Shi S  et al. Regulation of the epithelial Na+ channel by paraoxonase-2. J Biol Chem 292:15927-15938 (2017). PubMed: 28768768

Customer reviews and Q&As

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1-3 of 3 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PON2 antibody [EPR15295-82]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Xenografted tumor intercranial)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Xenografted tumor intercranial
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Anton Poral

Verified customer

Submitted Jul 31 2019

IHC - Wholemount abreview for Anti-PON2 antibody [EPR15295]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (normal and tumor sections)
Specification
normal and tumor sections
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 27 2015

Western blot abreview for Anti-PON2 antibody [EPR15295]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T, mouse embryonic fibroblasts)
Specification
293T, mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 29 2014

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