Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T, mouse embryonic fibroblasts)
Specification
293T, mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1xPBS+ 0.1% Tween20
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody,
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 40 kDa
Exposure
20 second(s)
Positive control
293T
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 29 2014