Recombinant Anti-PON2 antibody [EPR18547-2] - BSA and Azide free (ab228134)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18547-2] to PON2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-PON2 antibody [EPR18547-2] - BSA and Azide free
See all PON2 primary antibodies -
Description
Rabbit monoclonal [EPR18547-2] to PON2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): L-929 cells.
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General notes
ab228134 is the carrier-free version of ab192038.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18547-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. -
Tissue specificity
Widely expressed with highest expression in liver, lung, placenta, testis and heart. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 330260 Mouse
- Entrez Gene: 296851 Rat
- SwissProt: Q62086 Mouse
- SwissProt: Q6AXM8 Rat
- Unigene: 126984 Mouse
- Unigene: 460999 Mouse
- Unigene: 1100 Rat
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Alternative names
- A esterase 2 antibody
- A-esterase 2 antibody
- Aromatic esterase 2 antibody
see all
Images
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PON2 was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab192038 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: L-929 whole cell lysate 10 μg (Input).
Lane 2: ab192038 IP in L-929 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192038 in L-929 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192038).
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Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized L-929 (mouse connective tissue fibroblast) cell line labeling PON2 with ab192038 at 1/600 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192038).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab228134 has not yet been referenced specifically in any publications.