Overview

  • Product name

    Anti-PON2 antibody [EPR18547-2] - BSA and Azide free
    See all PON2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18547-2] to PON2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q62086

  • Positive control

    • Flow cytometry: L-929 cells.
  • General notes

    Ab228134 is the carrier-free version of ab192038. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab228134 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

Target

  • Function

    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
  • Tissue specificity

    Widely expressed with highest expression in liver, lung, placenta, testis and heart.
  • Sequence similarities

    Belongs to the paraoxonase family.
  • Post-translational
    modifications

    The signal sequence is not cleaved.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A esterase 2 antibody
    • A-esterase 2 antibody
    • Aromatic esterase 2 antibody
    • Paraoxonase 2 antibody
    • Paraoxonase nirs antibody
    • PON 2 antibody
    • PON2 antibody
    • PON2_HUMAN antibody
    • Serum aryldialkylphosphatase 2 antibody
    • Serum paraoxonase/arylesterase 2 antibody
    see all

Images

  • PON2 was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab192038 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: L-929 whole cell lysate 10 μg (Input).

    Lane 2: ab192038 IP in L-929 whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192038 in L-929 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192038).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized L-929 (mouse connective tissue fibroblast) cell line labeling PON2 with ab192038 at 1/600 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192038).

References

ab228134 has not yet been referenced specifically in any publications.

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