Key features and details
- Rapid, reversible protein dye for nitrocellulose, PVDF and cellulose acetate membranes.
- CAS Number: 6226-79-5
- Soluble in water
- Form / State: Solid
- Source: Synthetic
Product namePonceau S, Protein dye
DescriptionRapid, reversible protein dye for nitrocellulose, PVDF and cellulose acetate membranes.
- 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
- Acid Red 112
- C.I. 27195
Protein dye for the rapid, reversible staining of protein bands on nitrocellulose, PVDF and cellulose acetate membranes. Staining is reversible allowing further immunological detection. Negative stain which binds to the positively charged amino groups of the protein. Also binds non-covalently to non-polar regions in the protein.
(Note: Ponceau S is not suitable for use with nylon membranes.)
Chemical name3-Hydroxy-4-[[2-sulfo-4-[(4-sulfophenyl)azo]phenyl]azo]-2,7-naphthalenedisulfonic acid tetrasodium salt
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab146313 has not yet been referenced specifically in any publications.