Product namePonceau S, Protein dye
DescriptionRapid, reversible protein dye for nitrocellulose, PVDF and cellulose acetate membranes.
- 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
- Acid Red 112
- C.I. 27195
Protein dye for the rapid, reversible staining of protein bands on nitrocellulose, PVDF and cellulose acetate membranes. Staining is reversible allowing further immunological detection. Negative stain which binds to the positively charged amino groups of the protein. Also binds non-covalently to non-polar regions in the protein.
(Note: Ponceau S is not suitable for use with nylon membranes.)
Chemical name3-Hydroxy-4-[[2-sulfo-4-[(4-sulfophenyl)azo]phenyl]azo]-2,7-naphthalenedisulfonic acid tetrasodium salt
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Avin KG et al. Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney Disease. PLoS One 11:e0159411 (2016). Read more (PubMed: 27486747) »
- Goldman A et al. Detection of Proteins on Blot Membranes. Curr Protoc Protein Sci 86:10.8.1-10.8.11 (2016). Read more (PubMed: 27801518) »
- Ni D et al. Immunoblotting and Immunodetection. Curr Protoc Mol Biol 114:10.8.1-10.8.37 (2016). Read more (PubMed: 27038386) »
- Ni D et al. Immunoblotting and Immunodetection. Curr Protoc Immunol 114:8.10.1-8.10.36 (2016). Read more (PubMed: 27479660) »
- Tsai YH et al. Selective, rapid and optically switchable regulation of protein function in live mammalian cells. Nat Chem 7:554-61 (2015). Read more (PubMed: 26100803) »