Key features and details
- Rabbit polyclonal to Ponticulin
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-Ponticulin antibody
DescriptionRabbit polyclonal to Ponticulin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Dictyostelium discoideum
- WB: Recombinant Dictyostelium discoideum (Slime mold) Ponticulin protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab226000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
RelevanceFunction: Binds F-actin and nucleates actin assembly. Major high affinity link between the plasma membrane and the cortical actin network.
Cellular localizationCell Membrane
- SwissProt: P54660 Dictyostelium discoideum
- ponA antibody
All lanes : Anti-Ponticulin antibody (ab226000) at 1/500 dilution
Lane 1 : Recombinant Dictyostelium discoideum (Slime mold) Ponticulin protein at 0.08 µg
Lane 2 : Recombinant Dictyostelium discoideum (Slime mold) Ponticulin protein at 0.04 µg
Lane 3 : Recombinant Dictyostelium discoideum (Slime mold) Ponticulin protein at 0.02 µg
Lane 4 : Recombinant Dictyostelium discoideum (Slime mold) Ponticulin protein at 0.01 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226000 has not yet been referenced specifically in any publications.