Product nameAnti-POP4 antibody
See all POP4 primary antibodies
DescriptionRabbit polyclonal to POP4
SpecificityThis antibody reacts with POP4
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
The region of Human POP4 from which the exact immunogen sequence was derived is amino acids 172-220 EDRLKVIPKLNCVFTVETDGFISYIYGSKFQLRSSERSAKKFKAKGTIDL . The exact sequence is proprietary information.
- HepG2 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
RelevancePOP4 is a part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. It may function with RPP38 to coordinate the nucleolar targeting and/or assembly of RNase P.
Cellular localizationNucleus; nucleolus
- EC 188.8.131.52 antibody
- hPOP4 antibody
- processing of precursor 4, ribonuclease P/MRP family, (S. cerevisiae) antibody
Lane 2 : Anti-POP4 antibody (ab50948) at 1.25 µg/ml
Lane 1 : MARKER
Lane 2 : HepG2 cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
ab50948 at 4µg/ml staining POP4 in paraffin-embedded sections of human kidney tissue. Human alveolar cells are positively labelled (indicated with arrows). Magnification: 400x.
ab50948 has not yet been referenced specifically in any publications.