Overview

  • Product name

    Anti-POP5 antibody
  • Description

    Rabbit polyclonal to POP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human POP5 aa 113-163. The exact sequence is proprietary.
    Database link: Q969H6

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to POP5 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

8 - 15 µl/mg lysate

Target

  • Function

    Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP.
  • Sequence similarities

    Belongs to the eukaryotic/archaeal RNase P protein component 2 family.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPop5 antibody
    • pop5 antibody
    • POP5_HUMAN antibody
    • Ribonuclease P/MRP protein subunit POP5 antibody

Images

  • POP5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241199 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241199 at 1/400 dilution.

    Lane 1: ab241199 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

References

ab241199 has not yet been referenced specifically in any publications.

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