Overview

  • Product name

  • Description

    Mouse polyclonal to POPDC2/POP2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human POPDC2/POP2.
    Database link: NP_071418

  • Positive control

    • POPDC2/POP2 transfected 293T lysate.
  • General notes

     This product was previously labelled as POPDC2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab69176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Important for the maintenance of cardiac function. Plays a regulatory function in heart rate dynamics mediated, at least in part, through cAMP-binding and, probably, by increasing cell surface expression of the potassium channel KCNK2 and enhancing current density.
  • Tissue specificity

    Expressed predominantly in the heart and in the skeletal muscle.
  • Sequence similarities

    Belongs to the popeye family.
  • Cellular localization

    Membrane. Cell membrane, sarcolemma.
  • Information by UniProt
  • Database links

  • Alternative names

    • POP 2 antibody
    • POP2 antibody
    • POPD2_HUMAN antibody
    • POPDC 2 antibody
    • Popdc2 antibody
    • Popeye domain containing 2 antibody
    • Popeye domain containing protein 2 antibody
    • Popeye domain-containing protein 2 antibody
    • Popeye protein 2 antibody
    see all

Images

  • All lanes : Anti-POPDC2/POP2 antibody (ab69176) at 1/500 dilution

    Lane 1 : POPDC2/POP2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 40 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

References

ab69176 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 05 2017

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