Recombinant
RabMAb

Recombinant Anti-POR1 antibody [EPR11943] (ab180503)

Overview

  • Product name

    Anti-POR1 antibody [EPR11943]
    See all POR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11943] to POR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human POR1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P53365

  • Positive control

    • SH-SY5Y, MCF-7, HeLa and HepG2 whole cell lysate (ab7900); Human gastric carcinoma tissue; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11943
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/250 - 1/500.
ICC/IF 1/1000.
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30.

Target

  • Function

    Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
  • Sequence similarities

    Contains 1 AH domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosylation factor interacting protein 2 antibody
    • ADP-ribosylation factor-interacting protein 2 antibody
    • Arfaptin 2 antibody
    • Arfaptin-2 antibody
    • ARFIP2 antibody
    • ARFP2_HUMAN antibody
    • FLJ18046 antibody
    • FLJ18697 antibody
    • FLJ99239 antibody
    • Partner of RAC1 antibody
    • POR1 antibody
    • Protein POR1 antibody
    see all

Images

  • All lanes : Anti-POR1 antibody [EPR11943] (ab180503) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa

  • Western blot analysis of SH-SY5Y cell lysate (Lane 1) and Negative control (Lane 2) immunoprecipitated with ab180503 at 1/30 dilution. Rabbit TrueBlot®:Anti-Rabbit IgG HRP secondary antibody used at 1/1500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/150 dilution (red), compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.

  • Immunofluorescent analysis of  4% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/1000 dilution, followed by Goat anti rabbit IgG (Dylight 555) secondary antibody at 1/250 dilution. Counter stained with DAPI.

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling POR2 with ab180503 at 1/500 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

ab180503 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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