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    porcine-cytokine-antibody-array-a-10-targets-quantitative-ab197479.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479)

  • Datasheet
  • SDS
  • Protocol Booklet
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Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Assay Summary
  • Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Array Map
  • Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Standard Curve

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Overview

  • Product name

    Porcine Cytokine Antibody Array A (10 Targets) - Quantitative
    See all Cytokine Antibody Array antibody arrays
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Assay type

    Quantitative
  • Assay time

    6h 00m
  • Species reactivity

    Reacts with: Pig
  • Product overview

    ab197479 can be used for quantitative measurement of 10 Porcine Cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
    Targets names: IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12, GM-CSF, IFNγ, TGFβ1, TNFα. 


    Learn more about quantitative antibody arrays.

  • Notes

    Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. 


    Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. 


    For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. 


    Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. 


    Learn more about quantitative antibody arrays. 


    Learn more about membrane antibody arrays.


     

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 8 tests 22 tests 50 tests
    20X Wash Buffer I 2 x 30ml 3 x 30ml 6 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 2 x 30ml
    Cy3 equivalent dye-conjugated Streptavidin 1 x 5µl 2 x 5µl 4 x 5µl
    Porcine Cytokine Array A (10 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units
    Porcine Cytokine Array A (10 T) Glass Slide 1 slide 2 slides 4 slides
    Porcine Cytokine Array A (10 T) Lyophilized Cytokine Standard Mix 1 vial 1 vial 4 vials
    Sample Diluent 1 x 15ml 1 x 15ml 2 x 15ml
    Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 1 unit 2 units
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Antibody Arrays
    • Membrane antibody arrays
    • Cytokine antibody arrays

Images

  • Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Assay Summary
    Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Assay Summary

    Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Assay Summary

  • Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Array Map
    Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Array Map

    Array Map for Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479).

  • Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Standard Curve
    Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Standard Curve

    Standard Curve obtained with Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab197479? Please let us know so that we can cite the reference in this datasheet.

ab197479 has been referenced in 1 publication.

  • Ouyang A  et al. Chronic exercise training prevents coronary artery stiffening in aortic-banded miniswine: role of perivascular adipose-derived advanced glycation end products. J Appl Physiol (1985) 127:816-827 (2019). PubMed: 31295062

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