Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Porcine Cytokine Antibody Array A (10 Targets) - Quantitative
See all Cytokine Antibody Array antibody arrays -
Sample type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Quantitative -
Assay time
6h 00m -
Species reactivity
Reacts with: Pig -
Product overview
ab197479 can be used for quantitative measurement of 10 Porcine Cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12, GM-CSF, IFNγ, TGFβ1, TNFα. -
Notes
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
Learn more about quantitative antibody arrays.
Learn more about membrane antibody arrays.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 8 tests 22 tests 50 tests 20X Wash Buffer I 2 x 30ml 3 x 30ml 6 x 30ml 20X Wash Buffer II 1 x 30ml 1 x 30ml 2 x 30ml Cy3 equivalent dye-conjugated Streptavidin 1 x 5µl 2 x 5µl 4 x 5µl Porcine Cytokine Array A (10 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Porcine Cytokine Array A (10 T) Glass Slide 1 slide 2 slides 4 slides Porcine Cytokine Array A (10 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 4 vials Sample Diluent 1 x 15ml 1 x 15ml 2 x 15ml Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 1 unit 2 units -
Research areas
Images
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Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Assay SummaryPorcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Assay Summary
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Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Array MapArray Map for Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479).
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Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479) Standard CurveStandard Curve obtained with Porcine Cytokine Antibody Array A (10 Targets) - Quantitative (ab197479).
Protocols
References
This product has been referenced in:
- Ouyang A et al. Chronic exercise training prevents coronary artery stiffening in aortic-banded miniswine: role of perivascular adipose-derived advanced glycation end products. J Appl Physiol (1985) 127:816-827 (2019). Read more (PubMed: 31295062) »
