Overview

  • Product name

    Porcine PDGF-BB ELISA
    See all PDGF B kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 < 10%
    Inter-assay
    Sample n Mean SD CV%
    1 < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10 pg/ml
  • Range

    10 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 120.5 108% - 130%
    Serum 108.6 87% - 123%
    Plasma 112.3 98% - 115%

  • Assay time

    4h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human, Pig
  • Product overview

    The Porcine PDGF-BB ELISA kit (ab218297) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of porcine PDGF-BB in serum, plasma and cell culture supernatants. 


    This assay employs an antibody specific for porcine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab218297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Porcine PDGF-BB ELISA kit (ab218297) Standard curve.

Protocols

References

ab218297 has not yet been referenced specifically in any publications.

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