Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19741] to PORCN/PPN
- Suitable for: WB
- Reacts with: Human
Product nameAnti-PORCN/PPN antibody [EPR19741]
See all PORCN/PPN primary antibodies
DescriptionRabbit monoclonal [EPR19741] to PORCN/PPN
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PORCN/PPN aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H237
- WB: HepG2 and K562 whole cell lysates; Human fetal kidney tissue lysate.
This product was previously labelled as PORCN
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab201793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 52 kDa).|
FunctionModulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members.
Tissue specificityIsoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung.
Involvement in diseaseDefects in PORCN are the cause of focal dermal hypoplasia (FODH) [MIM:305600]; also known as Goltz Gorlin syndrome. A rare congenital ectomesodermal disorder characterized by a combination of skin defects, skeletal abnormalities, and ocular anomalies. Affected individuals have patchy dermal hypoplasia, often in a distribution pattern following the Blaschko lines, and areas of subcutaneous fat herniation or deposition of fat into the dermis. In addition, sparse and brittle hair, hypoplastic nails and papillomas have been described. Skeletal abnormalities usually comprise syndactyly, ectrodactyly, and brachydactyly, and in some cases osteopathia striata has been seen. Patients frequently have ocular anomalies, including microphthalmia/ anophthalmia, coloboma, pigmentary and vascularization defects of the retina. Dental abnormalities are often present.
Sequence similaritiesBelongs to the membrane-bound acyltransferase family. Porcupine subfamily.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
FormCellular component Endoplasmic reticulum, Membrane. Modulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members.
- DHOF antibody
- FODH antibody
- MG61 antibody
All lanes : Anti-PORCN/PPN antibody [EPR19741] (ab201793) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
Our immunogen covers all 5 isoforms but only isoform 5 (42kDa) is observed in our testing condition. This is consistent with the literature (PMID: 21768372).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201793 has not yet been referenced specifically in any publications.