Anti-POT1 antibody (ab229803)
Key features and details
- Rabbit polyclonal to POT1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-POT1 antibody
See all POT1 primary antibodies -
Description
Rabbit polyclonal to POT1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human POT1 aa 250-450.
Database link: Q9NUX5 -
Positive control
- IHC-P: Human skeletal muscle and testis tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the telombin family. -
Cellular localization
Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA. - Information by UniProt
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Database links
- Entrez Gene: 25913 Human
- Omim: 606478 Human
- SwissProt: Q95K48 Cynomolgus monkey
- SwissProt: Q9NUX5 Human
- Unigene: 31968 Human
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Alternative names
- DKFZP586D211 antibody
- hPot 1 antibody
- hPot1 antibody
see all
Images
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Paraffin-embedded human skeletal muscle tissue stained for POT1 with ab229803 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for POT1 with ab229803 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for POT1 (green) using ab229803 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229803 has not yet been referenced specifically in any publications.