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Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 387-417 of Human POTEE (NP_001077007.1).
Our Abpromise guarantee covers the use of ab108190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/100 - 1/500. Predicted molecular weight: 121 kDa.|
ab108190 staining POTEE in human ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/125 in PBS) for 24 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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