Overview

  • Product name

    Anti-POU Domain 2 antibody [6G6AC11]
  • Description

    Rat monoclonal [6G6AC11] to POU Domain 2
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC (PFA fixed), ICC/IFmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Drosophila stage 10 embryos.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    Purity: 97.47%
  • Clonality

    Monoclonal
  • Clone number

    6G6AC11
  • Isotype

    IgG2a
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab201325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    DNA-binding regulatory protein implicated in early development. Involved in neuronal cell fate decision. May act as an octamer-dependent activator of transcription. Could also play an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system. Expressed primarily during the first half of embryogenesis.
  • Cellular localization

    nucleus
  • Database links

    • Alternative names

      • CG12287 antibody
      • CG15486 antibody
      • CG15487 antibody
      • Didymous antibody
      • dOct2 antibody
      • dPOU-28 antibody
      • Miti mere antibody
      • Miti-mere antibody
      • pdm2 antibody
      • POU domain protein 2 antibody
      • POU domain protein 2, isoform B antibody
      • Protein didymous antibody
      see all

    Images

    • Immunocytochemistry analysis in neuroblast nuclei from Drosophila stage 10 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The POU Domain 2 stain used ab201325 at 9 µg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-rat at 1:400.

    References

    This product has been referenced in:

    • Bahrampour S  et al. Brain expansion promoted by polycomb-mediated anterior enhancement of a neural stem cell proliferation program. PLoS Biol 17:e3000163 (2019). Read more (PubMed: 30807568) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab201325.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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