Our Abpromise guarantee covers the use of ab66260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.


  • Function
    May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificity
    Brain. Seems to be specific to the retina.
  • Involvement in disease
    Defects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15) [MIM:602459]. DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox/POU domain protein 3C antibody
    • Brain-3C antibody
    • Brain-specific homeobox/POU domain protein 3C antibody
    • BRN 3C antibody
    • Brn-3C antibody
    • BRN3C antibody
    • class 4 antibody
    • DFNA 15 antibody
    • DFNA15 antibody
    • MGC138412 antibody
    • PO4F3_HUMAN antibody
    • POU class 4 homeobox 3 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 3 antibody
    • POU4F3 antibody
    • transcription factor 3 antibody
    see all


  • Anti-POU4F3 antibody (ab66260) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa
    Additional bands at: 34 kDa, 45 kDa, 64 kDa. We are unsure as to the identity of these extra bands.

    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling POU4F3 with ab66260 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.


This product has been referenced in:
  • Ishii J  et al. Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation. Pathol Int 64:415-22 (2014). Read more (PubMed: 25243889) »
  • Walshe TE  et al. TGF-ß signaling is required for maintenance of retinal ganglion cell differentiation and survival. Neuroscience 189:123-31 (2011). WB ; Rat . Read more (PubMed: 21664439) »
See all 2 Publications for this product

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