Key features and details
- Rabbit polyclonal to POU5F1B
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-POU5F1B antibody
DescriptionRabbit polyclonal to POU5F1B
Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Pig
Synthetic peptide within Human POU5F1B aa 214-242 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q06416
- WB: K562 cell lysate. IHC-P: Human testis tissue. Flow Cyt: K562 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.|
|WB||1/1000. Predicted molecular weight: 39 kDa.|
FunctionShows weak transcriptional activator activity.
Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
- Information by UniProt
- class 5 antibody
- Octamer-binding protein 3-like antibody
- Octamer-binding transcription factor 3-like antibody
Anti-POU5F1B antibody (ab230429) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg
Predicted band size: 39 kDa
Formalin-fixed, paraffin-embedded human testis tissue stained for POU5F1B using ab230429 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling POUF5F1B with ab230429 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230429 has not yet been referenced specifically in any publications.