Overview

  • Product name
    Anti-POU5F1B antibody
  • Description
    Rabbit polyclonal to POU5F1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Synthetic peptide within Human POU5F1B aa 214-242 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q06416

  • Positive control
    • WB: K562 cell lysate. IHC-P: Human testis tissue. Flow Cyt: K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 39 kDa.

Target

  • Function
    Shows weak transcriptional activator activity.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • class 5 antibody
    • Octamer-binding protein 3-like antibody
    • Octamer-binding transcription factor 3-like antibody
    • OTF3C antibody
    • OTF3P1 antibody
    • P5F1B_HUMAN antibody
    • POU5F1B antibody
    • POU5F1P1 antibody
    • POU5FLC20 antibody
    • POU5FLC8 antibody
    • Putative POU domain antibody
    • Putative POU domain, class 5, transcription factor 1B antibody
    • transcription factor 1B antibody
    see all

Images

  • Anti-POU5F1B antibody (ab230429) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

    Predicted band size: 39 kDa

  • Formalin-fixed, paraffin-embedded human testis tissue stained for POU5F1B using ab230429 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling POUF5F1B with ab230429 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230429 has not yet been referenced specifically in any publications.

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