- References (1)
Product namePP 1, Src family tyrosine kinase inhibitor
DescriptionPotent Src family tyrosine kinase inhibitor
Biological descriptionPotent Src family tyrosine kinase inhibitor. Inhibits a multitude of kinases (IC50 values are 0.05 (LCK), 0.52 (CSK), 0.64 (p38α) and 1.06 (CK1δ) µM). Inhibits Bcr-Abl kinase and induces apoptosis.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 25 mM and in ethanol to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Herpes simplex virus-2 regulates ER-alpha and HA via Src kinase in ECT-1 cells.
(Panel B) ECT-1 cells were treated with vehicle (DMSO) or Src inhibitor (SKI/PP1). ER-alpha was quantified using densitometry following Western blot analysis (ANOVA with Tukey’s multiple comparison; NT vs VEH, n.s.; NT vs SKI/PPI, p< .02; VEH vs SKI/PPI, p< .01).
ab11512 staining E cadherin in SW480 cells treated with PP1 (ab120859), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of PP1, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120859 (PP1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Tatton L et al. The Src-selective kinase inhibitor PP1 also inhibits Kit and Bcr-Abl tyrosine kinases. J Biol Chem 278:4847-53 (2003). Read more (PubMed: 12475982) »
- Bain J et al. The specificities of protein kinase inhibitors: an update. Biochem J 371:199-204 (2003). Read more (PubMed: 12534346) »
- Hanke JH et al. Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor. Study of Lck- and FynT-dependent T cell activation. J Biol Chem 271:695-701 (1996). Read more (PubMed: 8557675) »