Overview

  • Product name

  • Description

    Rabbit polyclonal to PP-T
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PP-T (internal sequence). The exact sequence is proprietary.
    Database link: P53041

  • Positive control

    • WB: HEK-293T and A431 whole cell extract. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
    Contains 3 TPR repeats.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36922 antibody
    • FLJ55954 antibody
    • OTTHUMP00000165834 antibody
    • PP-T antibody
    • PP5 antibody
    • PPP5 antibody
    • PPP5_HUMAN antibody
    • Ppp5c antibody
    • PPT antibody
    • Protein phosphatase 5, catalytic subunit antibody
    • Protein phosphatase T antibody
    • Serine/threonine protein phosphatase 5 antibody
    • Serine/threonine-protein phosphatase 5 antibody
    see all

Images

  • All lanes : Anti-PP-T antibody (ab227227) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    10% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PP-T (green) using ab227227 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

References

ab227227 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab227227.
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