Product nameAnti-PP-T antibody
See all PP-T primary antibodies
DescriptionRabbit polyclonal to PP-T
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
Recombinant fragment within Human PP-T (internal sequence). The exact sequence is proprietary.
Database link: P53041
- WB: HEK-293T and A431 whole cell extract. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
- Information by UniProt
- FLJ36922 antibody
- FLJ55954 antibody
- OTTHUMP00000165834 antibody
All lanes : Anti-PP-T antibody (ab227227) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PP-T (green) using ab227227 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
ab227227 has not yet been referenced specifically in any publications.