Overview

  • Product name

  • Description

    Rabbit polyclonal to PP-T
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human PP-T aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab27751, ab27752)

  • Positive control

    • Recombinant Human PP-T protein (ab91714) can be used as a positive control in WB. This antibody gave a positive result in the following lysates: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab26053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
    Contains 3 TPR repeats.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36922 antibody
    • FLJ55954 antibody
    • OTTHUMP00000165834 antibody
    • PP-T antibody
    • PP5 antibody
    • PPP5 antibody
    • PPP5_HUMAN antibody
    • Ppp5c antibody
    • PPT antibody
    • Protein phosphatase 5, catalytic subunit antibody
    • Protein phosphatase T antibody
    • Serine/threonine protein phosphatase 5 antibody
    • Serine/threonine-protein phosphatase 5 antibody
    see all

Images

  • All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution


    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

References

This product has been referenced in:

  • Norris-Mullins B  et al. Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major. Parasite 21:25 (2014). WB . Read more (PubMed: 24890370) »
  • Zhang Y  et al. Estrogen inhibits glucocorticoid action via protein phosphatase 5 (PP5)-mediated glucocorticoid receptor dephosphorylation. J Biol Chem 284:24542-52 (2009). WB ; Human . Read more (PubMed: 19586900) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting us. The homology of the immunogen with Xenopus PP5 is 93%. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080 Cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HT1080 Cell line
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 04 2010

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50000 cells
Specification
HT1080 Cell line
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jun 04 2010

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