Overview

  • Product name
  • Description
    Rabbit polyclonal to PP-T
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human PP-T aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab27751, ab27752)

  • Positive control
    • Recombinant Human PP-T protein (ab91714) can be used as a positive control in WB. This antibody gave a positive result in the following lysates: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab26053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
    Contains 3 TPR repeats.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36922 antibody
    • FLJ55954 antibody
    • OTTHUMP00000165834 antibody
    • PP-T antibody
    • PP5 antibody
    • PPP5 antibody
    • PPP5_HUMAN antibody
    • Ppp5c antibody
    • PPT antibody
    • Protein phosphatase 5, catalytic subunit antibody
    • Protein phosphatase T antibody
    • Serine/threonine protein phosphatase 5 antibody
    • Serine/threonine-protein phosphatase 5 antibody
    see all

Images

  • All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution


    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

References

This product has been referenced in:
  • Norris-Mullins B  et al. Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major. Parasite 21:25 (2014). WB . Read more (PubMed: 24890370) »
  • Zhang Y  et al. Estrogen inhibits glucocorticoid action via protein phosphatase 5 (PP5)-mediated glucocorticoid receptor dephosphorylation. J Biol Chem 284:24542-52 (2009). WB ; Human . Read more (PubMed: 19586900) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting us. The homology of the immunogen with Xenopus PP5 is 93%. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080 Cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HT1080 Cell line
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 04 2010

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50000 cells
Specification
HT1080 Cell line
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jun 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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