Product nameAnti-PP-T antibody
See all PP-T primary antibodies
DescriptionRabbit polyclonal to PP-T
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
Synthetic peptide corresponding to Human PP-T aa 150-250.
(Peptide available as
- Recombinant Human PP-T protein (ab91714) can be used as a positive control in WB. This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab34693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human PP-T peptide (ab27751).|
FunctionMay play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
- Information by UniProt
- FLJ36922 antibody
- FLJ55954 antibody
- OTTHUMP00000165834 antibody
All lanes : Anti-PP-T antibody (ab34693) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa (possible cross reactivity)
ab34693 detects a band of approximately 59 kDa which corresponds in size to PP-T. It also crossreacts with a band at 75 kDa in HeLa cell extract which we cannot identify.