Key features and details
- Rabbit polyclonal to PP-T
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PP-T antibody
See all PP-T primary antibodies
DescriptionRabbit polyclonal to PP-T
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
Synthetic peptide corresponding to Human PP-T aa 150-250.
(Peptide available as
- Recombinant Human PP-T protein (ab91714) can be used as a positive control in WB. This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab34693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human PP-T peptide (ab27751).|
FunctionMay play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
- Information by UniProt
- FLJ36922 antibody
- FLJ55954 antibody
- OTTHUMP00000165834 antibody
All lanes : Anti-PP-T antibody (ab34693) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa (possible cross reactivity)
ab34693 detects a band of approximately 59 kDa which corresponds in size to PP-T. It also crossreacts with a band at 75 kDa in HeLa cell extract which we cannot identify.
ab34693 has been referenced in 1 publication.
- Tukaj S et al. Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. PLoS One 8:e70496 (2013). PubMed: 23936217