Product nameAnti-PP-T antibody
See all PP-T primary antibodies
DescriptionRabbit polyclonal to PP-T
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human PP-T.
- Whole cell lysate from Hela cells, 293T cells and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionMay play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
- Information by UniProt
- FLJ36922 antibody
- FLJ55954 antibody
- OTTHUMP00000165834 antibody
All lanes : Anti-PP-T antibody (ab71150) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PP-T with ab71150 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of PP-T Lane 1: ab71150 at 3µg/mg whole cell lysate. Lane 2: IgG control IP Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Subsequent WB detection was performed using 1 µg/ml ab71150.
Chemiluminescence with an exposure time of 5 seconds.
ab71150 has not yet been referenced specifically in any publications.