Product nameAnti-PP-X antibody
See all PP-X primary antibodies
DescriptionRabbit polyclonal to PP-X
Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Rabbit
- Recombinant Human PP-X protein (ab114692) can be used as a positive control in WB. Bovine testes homogenate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab16475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 34 kDa.|
|ICC||1/500 - 1/1000.|
FunctionProtein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- PP X antibody
- PP-X antibody
- Pp4 antibody
All lanes : Anti-PP-X antibody (ab16475) at 1/10000 dilution (in PBS + 5% milk +0.1% Tween 20 for 1 hour at 25°C)
Lane 1 : HeLa whole cell lysate - mock transfected
Lane 2 : HeLa whole cell lysate - transfected with human siRNA against PP-X
Lysates/proteins at 100000 cells per lane.
All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/50000 dilution
Performed under non-reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Blocking Step: 5% Milk for 1 hour at 25°C
This product has been referenced in:
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). Read more (PubMed: 27391343) »
- Huang X et al. Protein phosphatase 4 plays dual roles during cell proliferation. Cell Prolif 49:219-35 (2016). Read more (PubMed: 27041735) »