The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/70.
1/1000 - 1/10000. Detects a band of approximately 42, 47 kDa (predicted molecular weight: 47 kDa).
Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
Placental-specific, but also associated with various malignant neoplasms.
Belongs to the ENDOU family. Contains 2 SMB (somatomedin-B) domains.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Immunohistochemical analysis of Paraffin-embedded Human placenta tissue labeling PP11 with ab185209 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot analysis of immunoprecipitation pellet from Human fetal skin lysate immunoprecipitated using ab185209 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.