Product nameAnti-PP2A-2 catalytic subunit antibody - C-terminal
DescriptionRabbit polyclonal to PP2A-2 catalytic subunit - C-terminal
Specificityab188253 recognized both PPA1 and PPA2 polypeptides in S. pombe because of their high amino acid similarity (~80% identity).
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Schizosaccharomyces pombe
Recombinant fragment corresponding to Schizosaccharomyces pombe PPA2 (C terminal).
Database link: P23636
- S. pombe cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab188253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 36 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
All lanes : Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253) at 1/1000 dilution
Lane 1 : Wild-type S. pombe cell extract
Lane 2 : ΔPPA1
Lane 3 : ΔPPA2
Lane 4 : Wild-type carrying a multicopy plasmid with PPA1 gene
Lane 5 : Wild-type carrying a multicopy plasmid with PPA2 gene
Lane 6 : Wild-type carrying a multicopy plasmid with ADH promoter ligated with PPA2 gene
Predicted band size: 36 kDa
The positions of PPA1 (36 kDa) and PPA2 (39 kDa) polypeptide bands are indicated.
Immunofluorescence analysis of S.pombe cells, labeling PPA2 with ab188253 (right). DNA staining with DAPI (left). 1. ΔPPA2. 2. wild-type. 3. wild-type overexpressing PPA2 (nmt-PPA2, 18hr). Bar, 10 µm.
ab188253 has not yet been referenced specifically in any publications.