Overview

  • Product name

    Anti-PP2A-2 catalytic subunit antibody - C-terminal
  • Description

    Rabbit polyclonal to PP2A-2 catalytic subunit - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab188253 recognized both PPA1 and PPA2 polypeptides in S. pombe because of their high amino acid similarity (~80% identity).
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Recombinant fragment corresponding to Schizosaccharomyces pombe PPA2 (C terminal).
    Database link: P23636

  • Positive control

    • S. pombe cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab188253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Images

  • All lanes : Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253) at 1/1000 dilution

    Lane 1 : Wild-type S. pombe cell extract
    Lane 2 : ΔPPA1
    Lane 3 : ΔPPA2
    Lane 4 : Wild-type carrying a multicopy plasmid with PPA1 gene
    Lane 5 : Wild-type carrying a multicopy plasmid with PPA2 gene
    Lane 6 : Wild-type carrying a multicopy plasmid with ADH promoter ligated with PPA2 gene

    Predicted band size: 36 kDa



    The positions of PPA1 (36 kDa) and PPA2 (39 kDa) polypeptide bands are indicated.

  • Immunofluorescence analysis of S.pombe cells, labeling PPA2 with ab188253 (right). DNA staining with DAPI (left). 1. ΔPPA2. 2. wild-type. 3. wild-type overexpressing PPA2 (nmt-PPA2, 18hr). Bar, 10 µm.

References

ab188253 has not yet been referenced specifically in any publications.

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