• Product name
    Anti-PP2A alpha + beta antibody [E155]
    See all PP2A alpha + beta primary antibodies
  • Description
    Rabbit monoclonal [E155] to PP2A alpha + beta
  • Host species
  • Specificity
    The immunogen sequence for ab32104 has 100% homology with the PP2A-beta protein.
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cyt, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Zebrafish
  • Immunogen

    Synthetic peptide within Human PP2A alpha + beta aa 250-350. The exact sequence is proprietary.

  • Positive control
    • IHC-P: Human pancreas tissue. WB: lysate of A431 cells (serum starved overnight) treated with EGF (100ng/ml) for 5-10 minutes at 37C (BD Biosciences).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 35 kDa.
IHC-P Use at an assay dependent concentration.
IP 1/100.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


Dot blot Use at an assay dependent concentration.


  • Function
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2A-beta antibody
    • PP2AA_HUMAN antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all


  • All lanes : Anti-PP2A alpha + beta antibody [E155] (ab32104) at 1/5000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysates,cells were treated with Pervanadate for 30 minutes at the final concentration of 1mg/ml
    Lane 3 : HeLa (human cervix adenocarcinoma) whole cell lysates,cells were treated with Pervanadate for 30 minutes at the final concentration of 1mg/ml Then the membrane was incubated with Alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), HRP conjugated)

    Predicted band size: 35 kDa

    Blocking and Diluting buffer and concentration:5% NFDM/TBST

    Exposure time:3 minutes

  • ICC/IF image of ab32104 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32104, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab32104 (2µg/ml) staining PP2A alpha  in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the islet of Langerhans.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PP2A alpha + beta with purified ab32104 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Dot blot was performed using ab32104 at 1/1000 dilution.Goat Anti-Rabbit IgG,(H+L),HRP conjugated (ab97051) was used as secondary antibody at 1/100000 dilution

    Lane 1:PP2A alpha + beta  phospho peptide

    Lane 2:PP2A alpha + beta non-phospho peptide

    Blocking and Diluting buffer and concentration:5% NFDM/TBST

    Exposure time:10 seconds

  • All lanes : Anti-PP2A alpha + beta antibody [E155] (ab32104) at 1/5000 dilution

    Lane 1 : untreated A431 cell lysate
    Lane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information)

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


This product has been referenced in:
  • Williams AP  et al. Investigation of PP2A and Its Endogenous Inhibitors in Neuroblastoma Cell Survival and Tumor Growth. Transl Oncol 12:84-95 (2019). Read more (PubMed: 30286326) »
  • Yuan C  et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing ß-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 1865:161-180 (2019). Read more (PubMed: 30389579) »
See all 32 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Te pido disculpas por la espera.

Respecto al anticuerpo ab32104, hemos realizado un BLAST comparando ambas secuencias, y existe un 77% de homología entre ellas. No se prevé por tanto que el anticuerpo pueda reconocerla.

¿De qué especie se trata? ¿En qué aplicación lo quieres usar?

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Thank you for your enquiry. I am sorry to hear you are having a problem with ab32104. To our knowledge, the antibody does not react with mouse. Therefore, we are unable to say, which of the two bands might be correct or if any of them is specific. I would recommend to use a positive control, as described on our datasheet and run it next to your sample. Doing this, you will be able to see, which band corresponds to the control band. Please let me know if this helps and do not hesitate to contact us for further advice.

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I have the regret to inform you that the scientist who tested the antibody informed me that the stimulation paradigm was confidential. I'm sorry I was unable to provide this information.

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