Recombinant Anti-PP2A alpha + beta antibody [Y119] (ab32141)


  • Product name
    Anti-PP2A alpha + beta antibody [Y119]
    See all PP2A alpha + beta primary antibodies
  • Description
    Rabbit monoclonal [Y119] to PP2A alpha + beta
  • Host species
  • Specificity
    This antibody detects both alpha and beta isoforms of PP2A.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PP2A alpha + beta (C terminal). The exact sequence is proprietary.

  • Epitope
    ab32141 reacts with an epitope located in the C terminal region of PP2A alpha and beta
  • Positive control
    • T47-D cell lysate and human breast carcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/250 - 1/500.
ICC 1/50.
IP 1/100.
Flow Cyt 1/20.


  • Function
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2A-beta antibody
    • PP2AA_HUMAN antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all


  • Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate

    Predicted band size: 35 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

  • ab32141,at a 1/250 dilution, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling PP2A alpha + beta (red) with unpurified ab32141 at dilution of 1/20. Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibodies.


This product has been referenced in:
  • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30871020) »
  • da Silva RA  et al. Laminar shear stress-provoked cytoskeletal changes are mediated by epigenetic reprogramming of TIMP1 in human primary smooth muscle cells. J Cell Physiol 234:6382-6396 (2019). Read more (PubMed: 30238981) »
See all 18 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


Thank you for your inquiry.

We generally expect a cross reaction between species of an antibody when the homology is 85% and higher. We consider this then worth testing.

Unfortunately, the immunogenof ab32141 and Neurospora crassa only show 78% homology. Therefore I cannot recommend this antibody for your purposes.

I also check our other Anti-PP2A alpha and beta antibodies.

They all also show a homology that is too low to expect a cross reaction, apart fromab1309.

ab1309 shows a 84% homology with Neurospora crassa. This is borderline, but might be worth testing.

Therefore, I can offer a discount off a future purchase if you buy ab1309 now, test it in Neurosporacrassaand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or the value off your next order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab1309 in Neurospora crassa. I will then send a discount code. This code must be issued before purchasing ab1309 so please wait for my reply before ordering.

2. Purchase ab1309 either by phone, fax, or online (

3. Test it in Neurospora crassa.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit:

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab1309 turns out to be unsuitable for Neurospora crassa, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at:

Read More
Western blot
Mouse Cell lysate - whole cell (Mouse testis)
Loading amount
25 µg
Mouse testis
Gel Running Conditions
Reduced Denaturing (15 %)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jul 18 2011

Mouse Cell lysate - whole cell (mouse testis)
Total protein in input
25 µg
mouse testis
Immuno-precipitation step
Protein G

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jul 14 2011

Immunocytochemistry/ Immunofluorescence
Human Cell (HT1080 Cell line)
HT1080 Cell line
Yes - 0.1% Triton X-100

Abcam user community

Verified customer

Submitted Jun 15 2010

Western blot
Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount
50000 cells
HT1080 Cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jun 04 2010

Western blot
Human Cell lysate - whole cell (A549 lung carcinoma)
Loading amount
40 µg
A549 lung carcinoma
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 06 2009

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