Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 lung carcinoma)
Loading amount
40 µg
Specification
A549 lung carcinoma
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Other product details

Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit alkaline phosphatase conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/5000

Detection

Detection method
BCIP/NBT
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 36 kDa

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Submitted Apr 06 2009

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