Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount
50000 cells
Specification
HT1080 Cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 2.5% Milk + 0.05% Tween20

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 34 kDa

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Submitted Jun 04 2010

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