Immunocytochemistry/ Immunofluorescence abreview for Anti-PP2A alpha and beta antibody [Y119]

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080 Cell line)
Specification
HT1080 Cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100

Other product details

Concentration
10 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 488 Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000

Additional data

Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in IF. This antibody has not been tested with the HT1080 cell line previously and your data suggest it may not be suitable for these cells.

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Submitted Jun 15 2010

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