Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse testis)
Loading amount
25 µg
Specification
Mouse testis
Gel Running Conditions
Reduced Denaturing (15 %)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 36 kDa

Additional data

Additional Notes
P2PA from mouse testis.
Line 1: Cells were solubilized using Brij-35 detergent
Line 2: Cells were solubilized using Triton-X100

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jul 18 2011

Sign up