Immunoprecipitation abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse testis)
Total protein in input
25 µg
Specification
mouse testis
Immuno-precipitation step
Protein G

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%TX-100, 10% glycerol, 1XPBS

Western Blot antibody

Name
Non-Abcam antibody was used: Goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Additional data

Additional Notes
PP2A was precipitated from mouse testis cells after lysis with Triton-X buffer. L = lysate, W = wash, IP = immunoprecipitation with anti PP2A Ab.

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jul 14 2011

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