Western blot abreview for Anti-PP2A alpha (methyl L309) antibody [2A10]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast)
Loading amount
25 µg
Specification
Mouse Embryonic Fibroblast
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBST

Secondary antibody

Name
Non-Abcam antibody was used: Sheep anti-mouse IgG, HRP linked
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: very weak, 43 kDa

Additional data

Additional Notes
It is a very good antibody but need to use with higher concentration 1:100 and ECL+.

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Submitted Jul 27 2009

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