Western blot abreview for Anti-PP2A alpha (methyl L309) antibody [2A10]

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Bands
Specific: 36 kDa
Exposure
10 second(s)

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Submitted Oct 15 2013

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