Western blot abreview for Anti-PP2A alpha + beta (methylation L309) antibody [2A10]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye­ 800 anti-mouse antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 36 kDa Non-specific: 100 kDa
Positive control
Whole cell lysate from MEF cells

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Submitted Jan 20 2017

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