Product nameAnti-PP2A-alpha antibody
See all PP2A-alpha primary antibodies
DescriptionRabbit polyclonal to PP2A-alpha
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
- Rat kidney tissue lysate, Mammary cancer sections
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.|
|ICC||Use a concentration of 0.5 - 1 µg/ml. In acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.|
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
- Information by UniProt
- PP2A alpha antibody
- PP2A-alpha antibody
- PP2AA_HUMAN antibody
All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/ml
Lane 1 : Rat kidney tissue Lysate
Lane 2 : Rat Liver tissue Lysate
Lane 3 : Human Placenta tissue Lysate
Lane 4 : Mouse Kidney tissue Lysate
Lane 5 : A431 Whole Cell Lysate
Lane 6 : HeLa Whole Cell Lysate
Lane 7 : 293T Whole Cell Lysate
Lane 8 : NIH Whole Cell Lysate
Lane 9 : PC12 Whole Cell Lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa
Cytoplasmic staining of PP2A in a formalin fixed, paraffin embedded section of mammary cancer, using ab106262 at a concentration of 2µg/ml.
This product has been referenced in:
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). Read more (PubMed: 30969990) »
- Umesalma S et al. RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growth. J Clin Invest 130:N/A (2019). Read more (PubMed: 30721156) »