• Product name

  • Description

    Rabbit polyclonal to PP2A-alpha
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide aa 6-20 (N terminal).


    Database link: P67775

  • Positive control

    • Rat kidney tissue lysate, Mammary cancer sections



Our Abpromise guarantee covers the use of ab106262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.
ICC Use a concentration of 0.5 - 1 µg/ml. In acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.


  • Function

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links

  • Alternative names

    • PP2A alpha antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Aalpha antibody
    • PP2Ac antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PPP2CA antibody
    • Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2, catalytic subunit, alpha isozyme antibody
    • Protein phosphatase 2A catalytic subunit, alpha isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • RPC antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all


  • All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/ml

    Lane 1 : Rat kidney tissue Lysate
    Lane 2 : Rat Liver tissue Lysate
    Lane 3 : Human Placenta tissue Lysate
    Lane 4 : Mouse Kidney tissue Lysate
    Lane 5 : A431 Whole Cell Lysate
    Lane 6 : HeLa Whole Cell Lysate
    Lane 7 : 293T Whole Cell Lysate
    Lane 8 : NIH Whole Cell Lysate
    Lane 9 : PC12 Whole Cell Lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 36 kDa

  • Cytoplasmic staining of PP2A in a formalin fixed, paraffin embedded section of mammary cancer, using ab106262 at a concentration of 2µg/ml.


This product has been referenced in:

  • Stafman LL  et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). Read more (PubMed: 30969990) »
  • Umesalma S  et al. RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growth. J Clin Invest 130:N/A (2019). Read more (PubMed: 30721156) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Human Cell lysate - whole cell (Huh7 liver)
Huh7 liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Dec 30 2014

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