Overview

  • Product name

  • Description

    Rabbit polyclonal to PP2A-alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment aa 1-231.
    Database link: P67775

  • Positive control

    • HeLa, HepG2, Molt 4 or Mouse brain whole lysates
      Cal27 xenograft
      Methanol fixed Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

Target

  • Function

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links

  • Alternative names

    • PP2A alpha antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Aalpha antibody
    • PP2Ac antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PPP2CA antibody
    • Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2, catalytic subunit, alpha isozyme antibody
    • Protein phosphatase 2A catalytic subunit, alpha isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • RPC antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

Images

  • Anti-PP2A-alpha antibody (ab137825) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Anti-PP2A-alpha antibody (ab137825) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 36 kDa

  • Confocal immunofluorescence analysis of methanol fixed HeLa cells, labelling PP2A alpha with ab137825 (Green) at 1/500 dilution. Alpha tubulin filaments were labelled with an alpha tubulin antibody (Red) at 1/2000.
  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft, labelling PP2A alpha with ab137825 at 1/100 dilution.

References

ab137825 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your patience in awaiting our reply while we gathered our data on this. It does not appear that this has been specifically tested for our products but comparing the immunogens of our PP2CA antibodies there is a strong possibility that each will react in PP2CB as well. Please let me know if I can be of further assistance.

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