Overview

  • Product name

    Anti-PP2A-alpha antibody - C-terminal
    See all PP2A-alpha primary antibodies
  • Description

    Rabbit polyclonal to PP2A-alpha - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide within Human PP2A-alpha (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P67775

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab226790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IP 1/100 - 1/500.

Target

  • Function

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links

  • Alternative names

    • PP2A alpha antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Aalpha antibody
    • PP2Ac antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PPP2CA antibody
    • Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2, catalytic subunit, alpha isozyme antibody
    • Protein phosphatase 2A catalytic subunit, alpha isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • RPC antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

Images

  • All lanes : Anti-PP2A-alpha antibody - C-terminal (ab226790) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • PP2A-alpha was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab226790. Western blot was performed from the immunoprecipitate using ab226790.

    Lane 1: HEK-293T whole cell extract (Input).

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab226790 IP in HEK-293T whole cell extract.

References

ab226790 has not yet been referenced specifically in any publications.

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