Anti-PP2A alpha + beta antibody (ab1309)
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Overview
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Product nameAnti-PP2A alpha + beta antibody
See all PP2A alpha + beta primary antibodies -
DescriptionGoat polyclonal to PP2A alpha + beta
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Host speciesGoat
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SpecificityThis antibody will recognise both alpha and beta isoforms, which are almost identical.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human PP2A alpha + beta aa 297-309 (C terminal).
Sequence:C-EPHVTRRTPDYFL
(Peptide available asab22802) -
Positive control
- Recombinant Human PP2A-alpha protein (ab114424) can be used as a positive control in WB.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab1309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human PP2A alpha + beta peptide (ab22802). |
Target
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FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
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Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
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Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
- Information by UniProt
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Database links
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 397136 Pig
- Entrez Gene: 397656 Pig
- Entrez Gene: 24672 Rat
- Entrez Gene: 24673 Rat
see all -
Alternative names
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
see all
Images
Protocols
Datasheets and documents
References
ab1309 has not yet been referenced specifically in any publications.