Overview

  • Product name
    Anti-PP2A alpha + beta antibody
    See all PP2A alpha + beta primary antibodies
  • Description
    Rabbit polyclonal to PP2A alpha + beta
  • Host species
    Rabbit
  • Specificity
    ab137849 does cross react with PP2A beta
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human PP2A-alpha aa 247-259.

  • Positive control
    • 293T, A431, H1299, HeLaS3, Molt4, Raji and mouse brain lysates; HeLa cell; A549 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab137849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.

Target

  • Function
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2A-beta antibody
    • PP2AA_HUMAN antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

Images

  • All lanes : Anti-PP2A alpha + beta antibody (ab137849) at 1/1500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Molt4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Anti-PP2A alpha + beta antibody (ab137849) at 1/10000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cell labeling PP2A alpha with ab137849 (green) at a 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at a 1/2000 dilution.
  • Immunohistochemical analysis of paraffin embedded A549 xenograft, labeling PP2A alpha using ab137849 at a 1/100 dilution.

References

ab137849 has not yet been referenced specifically in any publications.

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