Key features and details
- Rabbit polyclonal to PP2A B subunit
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-PP2A B subunit antibody
DescriptionRabbit polyclonal to PP2A B subunit
Based on sequence homology we believe this antibody will detect the following isoforms of the PP2A B subunit. The immunogen sequence is 100% identical to Q16537; epsilon isoform (PPP2R5E), Q13362; gamma isoform (PPP2R5C), Q14738; delta isoform (PPP2R5D) and Q15173 beta isoform (PPP2R5B), and 92% identical to Q15172; alpha isoform (PPP2R5A). If the isoform you are interested in is not listed, please contact our technical support team on email@example.com who will be happy to check that for you.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PP2A B subunit aa 352-365 conjugated to Keyhole Limpet Haemocyanin (KLH). The sequence is also 100% identical to Q13362 (PPP2R5C), Q14738 (PPP2R5D) and Q15173 (PPP2R5B), and 92% identical to Q15172 (PPP2R5A).
Database link: Q16537
- WB: Rat brain extract and Recombinant Human PPP2R5D protein (ab114547)
A protein phosphatase is a phosphatase enzyme that removes a phosphate group from the phosphorylated amino acid residue of its substrate protein. Protein phosphorylation is one of the most common forms of reversible protein posttranslational modification (PTM), with up to 30% of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyse the transfer of a γ-phosphate from ATP to specific amino acids on proteins. Several hundred PKs exist in mammals and are classified into distinct super-families. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 79.3, 16.9 and 3.8% respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. The largest class of PPs is the phosphoprotein phosphatase (PPP) family comprising PP1, PP2A, PP2B, PP4, PP5, PP6 and PP7, and the protein phosphatase Mg2+ or Mn2+-dependent (PPM) family, composed primarily of PP2C.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesPurified by Ammonium sulfate precipitation.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
RelevanceSerine/threonine-protein phosphatase 2A 56 kDa regulatory subunit. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. There is an alpha isoform, a beta isoform, a gamma isoform, a delta isoform and an epsilon isoform.
- KIAA0044 antibody
- PP2A B subunit isoform B'-alpha antibody
- PP2A B subunit isoform B'-beta antibody
ab16448 has been referenced in 1 publication.
- Li P et al. Tristetraprolin attenuates brain edema in a rat model of cerebral hemorrhage. Brain Behav 9:e01187 (2019). PubMed: 30729695