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Synthetic peptide corresponding to Human PP2A beta.
Database link: P62714
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab168371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PPP2CB knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. Ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Detection of PPP2CB by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168371 at 1/10 dilution.
For WB detection ab168371 at 1/10000 dilution was used.
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow Cytometric analysis of permeabilized NIH 3T3 cells labeling PPP2CB with ab168371 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of 293T cells labeling PP2A beta with ab168371 at 1/50 dilution.
ab168371 has not yet been referenced specifically in any publications.