Overview

  • Product name

  • Description

    Rabbit polyclonal to PPA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PPA2 aa 25-75. The exact sequence is proprietary.
    Database link: Q9H2U2

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity

    Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.
  • Sequence similarities

    Belongs to the PPase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSPC124 antibody
    • Inorganic pyrophosphatase 2 antibody
    • Inorganic pyrophosphatase 2, mitochondrial antibody
    • IPYR2_HUMAN antibody
    • mitochondrial antibody
    • Ppa2 antibody
    • PPase 2 antibody
    • PPase2 antibody
    • Pyrophosphatase (inorganic) 2 antibody
    • Pyrophosphatase SID6 306 antibody
    • Pyrophosphatase SID6-306 antibody
    • Pyrophosphate phospho-hydrolase 2 antibody
    • SID6 306 antibody
    see all

Images

  • All lanes : Anti-PPA2 antibody (ab226378) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • PPA2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226378 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226378 at 1 µg/ml.

    Lane 1: ab226378 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226378 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226378.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up