Product nameAnti-PPA2 antibody
See all PPA2 primary antibodies
DescriptionRabbit polyclonal to PPA2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PPA2 aa 25-75. The exact sequence is proprietary.
Database link: Q9H2U2
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226378 was affinity purified using an epitope specific to PPA2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 38 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityDetected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.
Sequence similaritiesBelongs to the PPase family.
- Information by UniProt
- HSPC124 antibody
- Inorganic pyrophosphatase 2 antibody
- Inorganic pyrophosphatase 2, mitochondrial antibody
All lanes : Anti-PPA2 antibody (ab226378) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
PPA2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226378 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226378 at 1 µg/ml.
Lane 1: ab226378 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226378 has not yet been referenced specifically in any publications.