Key features and details
- Rabbit polyclonal to PPA2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PPA2 antibody
See all PPA2 primary antibodies
DescriptionRabbit polyclonal to PPA2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: HeLa whole cell lysate; Mouse kidney and liver lysates; Rat kidney and liver lysates. IHC-P: Human prostate tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|IHC-P||1/200 - 1/500.|
Tissue specificityDetected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.
Sequence similaritiesBelongs to the PPase family.
- Information by UniProt
- HSPC124 antibody
- Inorganic pyrophosphatase 2 antibody
- Inorganic pyrophosphatase 2, mitochondrial antibody
All lanes : Anti-HOXD1 antibody (ab235103) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat liver lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse liver lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Paraffin-embedded human protate tissue stained for PPA2 using ab235102 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235102 has not yet been referenced specifically in any publications.