Overview

  • Product name

    Anti-PPA2 antibody [EPR13086]
    See all PPA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13086] to PPA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PPA2 aa 1-100. The exact sequence is proprietary.
    Database link: Q9H2U2

  • Positive control

    • WB: Human fetal kidney mouse and rat heart tissue lysates and 293T, U87-MG and HepG2 cell lysates. IHC-P: Human brain and kidney tissues. IP: HepG2 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Tissue specificity

      Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.
    • Sequence similarities

      Belongs to the PPase family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • HSPC124 antibody
      • Inorganic pyrophosphatase 2 antibody
      • Inorganic pyrophosphatase 2, mitochondrial antibody
      • IPYR2_HUMAN antibody
      • mitochondrial antibody
      • Ppa2 antibody
      • PPase 2 antibody
      • PPase2 antibody
      • Pyrophosphatase (inorganic) 2 antibody
      • Pyrophosphatase SID6 306 antibody
      • Pyrophosphatase SID6-306 antibody
      • Pyrophosphate phospho-hydrolase 2 antibody
      • SID6 306 antibody
      see all

    Images

    • All lanes : Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 38 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-PPA2 antibody [EPR13086] (ab180859) at 1/10000 dilution (purified) + HepG2 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 38 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (purified) + 293T whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 38 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with purified ab180859 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab180859 (purified) at 1/80 immunoprecipitating PPA2 in HepG2 whole cell lysate.

      Lane 1 (input): HepG2 whole cell lysate (10µg)

      Lane 2 (+): ab180859 + HepG2 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180859 in HepG2 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal kidney lysate
      Lane 2 : 293T lysate
      Lane 3 : U87-MG lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180859 at a diltuion of 1/10.

    References

    ab180859 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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