Recombinant Anti-PPA2 antibody [EPR13087(B)] (ab177935)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13087(B)] to PPA2
- Suitable for: IP, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PPA2 antibody [EPR13087(B)]
See all PPA2 primary antibodies -
Description
Rabbit monoclonal [EPR13087(B)] to PPA2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, HepG2, human fetal kidney, U87-MG and 293T lysates; 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13087(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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Notes |
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IP
1/10 - 1/100. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Tissue specificity
Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium. -
Sequence similarities
Belongs to the PPase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 27068 Human
- Omim: 609988 Human
- SwissProt: Q9H2U2 Human
- Unigene: 654957 Human
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Alternative names
- HSPC124 antibody
- Inorganic pyrophosphatase 2 antibody
- Inorganic pyrophosphatase 2, mitochondrial antibody
see all
Images
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All lanes : Anti-PPA2 antibody [EPR13087(B)] (ab177935) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 5 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
Intracellular flow cytometric analysis of permeabilized 293 cellslabeling PPA2 with ab177935 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).
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Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab177935 has been referenced in 3 publications.
- Guimier A et al. PPA2-associated sudden cardiac death: extending the clinical and allelic spectrum in 20 new families. Genet Med 23:2415-2425 (2021). PubMed: 34400813
- Kennedy H et al. Sudden Cardiac Death Due to Deficiency of the Mitochondrial Inorganic Pyrophosphatase PPA2. Am J Hum Genet 99:674-682 (2016). PubMed: 27523597
- Guimier A et al. Biallelic PPA2 Mutations Cause Sudden Unexpected Cardiac Arrest in Infancy. Am J Hum Genet 99:666-673 (2016). PubMed: 27523598