Overview

  • Product name

    Anti-PPA2 antibody [EPR13087(B)]
    See all PPA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13087(B)] to PPA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPA2 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H2U2

  • Positive control

    • Human fetal liver, HepG2, human fetal kidney, U87-MG and 293T lysates; 293 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Tissue specificity

      Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.
    • Sequence similarities

      Belongs to the PPase family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • HSPC124 antibody
      • Inorganic pyrophosphatase 2 antibody
      • Inorganic pyrophosphatase 2, mitochondrial antibody
      • IPYR2_HUMAN antibody
      • mitochondrial antibody
      • Ppa2 antibody
      • PPase 2 antibody
      • PPase2 antibody
      • Pyrophosphatase (inorganic) 2 antibody
      • Pyrophosphatase SID6 306 antibody
      • Pyrophosphatase SID6-306 antibody
      • Pyrophosphate phospho-hydrolase 2 antibody
      • SID6 306 antibody
      see all

    Images

    • All lanes : Anti-PPA2 antibody [EPR13087(B)] (ab177935) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : U87-MG lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    • Flow cytometric analysis of permeabilized 293 cells labeling PPA2 with ab177935 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    • Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.

    References

    This product has been referenced in:

    • Kennedy H  et al. Sudden Cardiac Death Due to Deficiency of the Mitochondrial Inorganic Pyrophosphatase PPA2. Am J Hum Genet 99:674-682 (2016). Read more (PubMed: 27523597) »
    • Guimier A  et al. Biallelic PPA2 Mutations Cause Sudden Unexpected Cardiac Arrest in Infancy. Am J Hum Genet 99:666-673 (2016). Read more (PubMed: 27523598) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177935.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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